Information about samples is digitally registered in BIOBYTE®, and pseudonymized. BIOBYTE® generates barcode labels and enables sample tracking.
Characteristics of Biological Material for Multiple Myeloma (June 2017)
CD138+ cells: From 2008, isolation of cells was based on preparation of mononuclear cells from bone marrow and isolation of cancer cells by antibody and magnetic beads (RoboSep™, Stem Cell Technology). Samples are stored in aliquots of 0.7 mill cells (dry pellet) and 2 mill cells (DMSO, viable cells).
Cytospin slides are purified (>90 %) myeloma cells that are centrifuged down on a glass plate. The preparation is suitable for FISH analyses.
CD138- cells: This preparation contains the CD138- fraction, which is left after isolation of CD138+ cells and is stored in aliquots of 10 mill cells.
Mononuclear cells from the bone marrow, was the final preparation of cancer cells until 2008. Mononuclear cells were isolated from bone marrow by density gradient centrifugation. Purity of cancer cells (20-90 %) is dependent on the percentage of plasma cells in the aspirate.