The Prostate Biobank at Oslo University Hospital (Regional Ethical Committee for medical and healthcare research ethics Ref. 28144) is annually collecting formalin-fixed paraffin-embedded tissue samples from approximately 250 patients undergoing radical prostatectomy.
| FF tissue | Blood | Serum | Plasma | Germline DNA | |
| Total | 2794 | 1171 | 1290 | 1290 | 1280 |
Blood samples were routinely collected in the period 2006-2013. Matched serum and FF samples are available from more than 790 patients.
|
PSA |
PSA <10 |
10< PSA >20 |
PSA >20 |
Total |
|
n= |
751 |
370 |
86 |
1207 |
|
Grade Group biopsy |
1 |
2 |
3 |
4 |
5 |
Total |
|
n= |
508 |
413 |
140 |
129 |
17 |
1207 |
|
Grade Group RP |
1 |
2 |
3 |
4 |
5 |
Total |
|
n= |
381 |
476 |
219 |
94 |
31 |
1207 |
| pT-stage | pT1-pT2 | pT3a | pT3b - pT4 | Total | ||
| n= | 714 | 384 | 105 | 1207 |
Initially, 1-2 FF tissue cores were sampled from each prostatectomy specimen. In 2011, the Prostate Biobank implemented the “Multiple core protocol” allowing to address prostate cancer heterogeneity. 5-7 cores are collected per prostatectomy specimen. More than 95% of the samples are snap frozen within 15 minutes after arrival to the Department of Pathology. The average RNA Integrity Number (RIN) value based on Allprep DNA/RNA/miRNA Universal kit (Qiagen, Venlo, Netherlands) RNA extraction of 207 cores was 9.7 #.
Serum, plasma, and fresh frozen tissue as well as DNA are stored in 2D labeled tubes at minus 80 degrees Celsius in freezers with automatic, digital temperature logging. All samples are registered in the eBiobank system at Oslo University Hospital (OUS) and the clinical data in MedInsight.
The Research Biobank for Cancer has been responsible for the collection of biological samples from prostate cancer patients treated at OUS since fall 2019. Before that, biological samples were collected by The Prostate Biobank.
# Strømme et al., Cancer Gene Therapy 2022